中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (14): 2291-2296.doi: 10.3969/j.issn.2095-4344.2017.14.026

• 生物材料综述 biomaterial review • 上一篇    

微加工技术在细胞形状和排列控制中的应用

刘晓艺1,2,李 珊1,2,赵 峰1,何静雯1,孙 艳1,2   

  1. 1北京航空航天大学生物与医学工程学院生物力学与力生物学教育部重点实验室,北京市  100191;2中国科学院上海微系统与信息技术研究所,传感技术联合国家重点实验室,上海市  200050
  • 收稿日期:2017-04-01 出版日期:2017-05-18 发布日期:2017-06-10
  • 通讯作者: 孙艳,博士,硕士生导师,副教授,北京航空航天大学生物与医学工程学院,北京市 100191
  • 作者简介:刘晓艺,女,1993年生,河北省魏县人,汉族,北京航空航天大学生物与医学工程学院在读硕士,主要从事微加工技术控制细胞形状,诱导细胞排列方面的研究。
  • 基金资助:

    国家自然科学基金(31470942)

Application of micro-scale technologies in engineering cell geometry and alignment

Liu Xiao-yi 1, 2, Li Shan 1, 2, Zhao Feng1, He Jing-wen1, Sun Yan 1, 2   

  1. 1 Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; 2 State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China
  • Received:2017-04-01 Online:2017-05-18 Published:2017-06-10
  • Contact: Sun Yan, M.D., Master’s supervisor, Associate professor, Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China
  • About author:Liu Xiao-yi, Studying for master’s degree, Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31470942

摘要:

文章快速阅读

 

文题释义:
干剥离技术
:是将生物相容性良好的分子沉积在基底上形成薄膜并通过离子刻蚀和光刻蚀使其表面图案化,然后在该复合结构表面孵育并吸附具有生物活性的分子,最后将薄膜从基底剥离留下吸附分子,则基底表面被选择性地化学修饰。


背景:精确控制细胞形状、实现细胞体外排列对细胞生物学、组织工程和再生医学研究及应用均有极其重要的意义,而目前对控制细胞几何形状和诱导体外排列的方法缺乏系统性的研究和表述。
目的:总结并讨论表面化学修饰技术、表面拓扑学改造技术加工制备细胞生长基底的方法,以及实现细胞形状和排列控制的可行性和最新进展。
方法:作者检索1995至2017年百链云数据库和PubMed数据库中与微加工方法对细胞形状和体外排列影响相关的文献,并进行系统整理、归纳总结和分析。
结果与结论:随着微纳米加工技术的发展和对细胞生物学行为的深入研究,表面化学修饰、表面拓扑学改造、纤维支架等方法逐步被建立,以实现细胞形状的精确控制和体外排列。表面微图案化、微柱、微孔阵列等拓扑结构能够精确控制细胞几何形状,而基底表面拓扑形貌对细胞排列占据更重要的支配作用,可将基底表面化学修饰作用和拓扑形貌作用相结合更好地实现细胞体外排列。合适的基底结构可用于精确控制细胞的形状、调控干细胞的分化抑或诱导细胞的定向排列、促进细胞融合和分化等。

ORCID: 0000-0001-8025-3769(刘晓艺)

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

关键词: 生物材料, 组织工程, 细胞排列, 细胞形状, 微接触印刷技术, 干剥离技术, 沟槽阵列, 微柱阵列, 微孔阵列, 微波阵列, 纤维支架, 国家自然科学基金

Abstract:

BACKGROUND: It is of great significance for the research of cell biology, tissue engineering and regenerative medicine to precisely control cell geometry and alignment in vitro. However, there is a lack of systematic study and description of the methods for controlling cell geometry and inducing alignment in vitro.
OBJECTIVE: To summarize the methods of surface chemical treatment technology and surface topographical technology to prepare the substrate for cell growth and to discuss the feasibility and the latest progress in cell shape control and cell alignment.
METHODS: The author performed a data retrieval of PubMed and Bailianyun databases from 1995 to 2017 to search the articles addressing the effects of micro-scale technologies on cell geometry and alignment in vitro, and reviewed the literatures systematically.
RESULTS AND CONCLUSION: With the development of micro-scale technologies and in-depth study on cell behaviors, surface chemical treatment, surface topographical transformation and fibrous scaffolds are gradually developed to precisely control cell geometry and alignment. The surface micropatterning, pillar and pit arrays can precisely control cell geometry, and the topographical patterning dominates cell alignment over the chemical patterns. Surface chemical treatment and topographical patterning can also be combined to preferably engineer cell alignment in vitro. The suitable substrate can be used to control cell geometry precisely, regulate the differentiation of stem cells or induce cell alignment, and promote cell fusion and differentiation.

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

Key words: Cell Biology, Cell Shape, Tissue Engineering

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